Nucleotide sequence of a nuc gene encoding the thermonuclease ofStaphylococcus intermedius

Nucleotide sequence of a nuc gene encoding the thermonuclease of Staphylococcus intermedius.

A recombinant plasmid, pIP1607, containing the thermonuclease gene (nuc) of S.intermedius was detected in a pUC18 library, constructed by cloning cellular DNA of S.intermedius strain LRA076 partially digested with So«3A in the BamHl site. E.coli strain TGI was used as a recipient for DNA transformation. The screening method consisted in replica-plating the ampicillinresistant colonies on DNA-to...

Nucleotide Sequence of Gene Encoding Capsid Protein VPI of Foot-and-Mouth Disease Virus / Type O1 Iran

Nucleotide sequence of cDNA encoding for preprochymosin in native goat (Capra hircus) from Iran

Prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. In the present investigation we report sequence of cDNA encoding goat ( Capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. As bovine prochymosin, the caprine prochymosin cDNA encodes 365 amino ...

Nucleotide sequence of the gene encoding the matrix protein of a recent measles virus isolate.

The sequence of the M gene of the Hu2 strain of measles virus has been determined. In the coding region of the gene, six nucleotide replacements had occurred with respect to the sequence of the M gene of the Edmonston strain. Two of these were silent and only four led to amino acid replacements in the protein sequence even though in SDS-PAGE the mobility of the protein would indicate an Mr incr...

Computational Prediction of the Effects of Single Nucleotide Polymorphisms of the Gene Encoding Human Endothelial Nitric Oxide Synthase

ABSTRACT           Background and Objective: Genetic variations in the gene encoding endothelial nitric oxide synthase (eNOS) enzyme affect the susceptibility to cardiovascular disease. Identification of the way these changes affect eNOS structure and function in laboratory conditions is difficult and time-consuming. Thus, it seems essential to ...